Separation of Cypermethrin Diastereomers by Normal Phase Liquid Chromatography

Abstract

Insecticides cause great environmental problems. We studied the separation of cypermethrin diastereomers. Cypermethrin has three asymmetric carbon atoms; it has four diastereomers. Research with reversed phase high performance liquid chromatography was unsuccessful. For the separation, we applied normal phase high performance liquid chromatography. In a systematic study, the best column was selected and the mobile phase was optimized. When we used acetonitrile as a polar modifier in the mobile phase, we found that the behaviour of acetonitrile is different in the separation of the four diastereomers from the other modifiers.

Keywords:

• Diastereomers
• Cypermethrin
• NPLC
• Organic modifiers
• Acetonitrile

Acknowledgment

This paper, in part, appeared in the Balaton Symposium 2005. Sept. 7–9, 2005.