Abstract
In this study, we reported a high throughout isolation procedure of pinoresinol diglucoside (PDG) and liriodendrin (SDG) from the traditional Chinese medical plant Eucommia ulmoides Oliv. by high speed countercurrent chromatography. The solvent system consisted of n‐butanol‐acetic acid‐water(4∶1∶5, v/v/v), in which the lower phase was used as the mobile phase at a flow‐rate of 2.0 mL · min−1 in the head to tail elution mode. The method yielded 21 mg of PDG at 96.7% purity and 16 mg of liriodendrin at 95.5% purity from 200 mg of crude extract in a one step. The purity and identity of the isolated compounds were checked by high performance liquid chromatography (HPLC) analysis, in combination with mass spectrometry (MS), infrared spectrum (IR), and NMR measurements.