Analysis of Melatonin in Dosage Formulation by Capillary Electrophoresis

Abstract

A simple, fast, and reproducible capillary electrophoretic method is developed and validated for analysis of melatonin in pharmaceutical preparations. The CE method was developed by using a fused silica capillary (60 cm×50 µm I.D.), phosphate buffer (50 mM, 3.0 pH) as the background electrolyte (BGE), 20 kV applied voltage with UV detection at 214 nm, and at a working temperature of 23±1°C. Linearity was observed in the concentration range from 10 to 100 µg/L with a correlation coefficient (R²) of 0.9996. The limits of detection and quantification achieved were 10 and 15 µg/mL, respectively. The percentage recovery of melatonin from pharmaceutical preparations was 95.0. Validation parameters prove the precision of the method and its applicability for the determination of melatonin in pharmaceutical tablet formulations.

Keywords:

• Melatonin
• Capillary electrophoresis
• Pharmaceutical analysis
• Pharmaceutical tablet formulation