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Abstract
A new and simple liquid chromatographic method using a column packed with poly(styrene‐divinylbenzene) and pulsed electrochemical detection on a gold electrode for the determination of vertilmicin sulfate (new drug), micronomycin sulfate, etimicin sulfate, and sisomicin sulfate and their related substances was developed. The mobile phase consisted of an aqueous solution of 38 g L−1 sodium sulfate, 0.4 g L−1 sodium octanesulfonate, 13 mL L−1 (10 mL L−1 for micronomycin) tetrahydrofuran, and 50 mL L−1 0.2 M phosphate buffer (pH=3). The effects of the different chromatographic parameters on the separation were investigated. The specificity, assay linearity, low level assay linearity, and precision of assay were also investigated.