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Journal of Liquid Chromatography & Related Technologies Volume 30, 2007 - Issue 8
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Original Articles

Refolding of Mis-folded Recombinant Cytochrome c3 with Strong Cation Exchange Chromatography

Yehua Shen Department of Chemistry, Institute of Drug Discovery, Northwest University, Xi'an, ChinaCorrespondence[email protected]
,
Yuki Takayama Institute for Protein Research, Osaka University, Osaka, Japan; Institute for Bioinformatic Research and Development, Japan Science and Technology Agency, Tokyo, Japan
,
Yinmao Wei Department of Chemistry, Institute of Drug Discovery, Northwest University, Xi'an, China
,
Erisa Harada Institute for Protein Research, Osaka University, Osaka, Japan
,
Eiichi Nakatani Institute for Protein Research, Osaka University, Osaka, Japan
&
Hideo Akutsu Institute for Protein Research, Osaka University, Osaka, Japan
Pages 1051-1059 | Received 14 Sep 2006, Accepted 06 Nov 2006, Published online: 13 Apr 2007
 
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Abstract

Mis‐folded recombinant cytochrome c 3 (rcyt c 3) formed in the purification process was refolded with strong cation exchange chromatography, with tri‐gradient of salt, urea, and pH in the mobile phase. The optimal concentration of urea was found to be 6.0 mol/L in mobile phase to refold the mis‐folded rcyt c 3 under the experimental conditions. The recovery of the correctly refolded rcyt c 3 was calculated to be 57.0%.

Acknowledgment

The authors are grateful for the financial support from The Education Deparment of Shaanxi Province of China (No. 05JC30) and The Science & Technology Department of Shaanxi Province of China (No. 2005K10‐G1 (8)).

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