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Abstract
A rapid HPLC method for the identification of xanthophylls in ethanol extracts of corn was developed. This method requires only the extraction of xanthophylls using aqueous ethanol followed by injection of the microfiltered extract onto a C30 column. The solvent system consists of pure methanol and MTBE. Initial mobile phase concentration, gradient slope, and flow rate were optimized to reduce analysis times by 60% compared to the existing method while maintaining the minimum desired resolution.
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Acknowledgments
This work was supported by the Illinois Corn Marketing Board, Bloomington, IL, and the U. S. Department of Agriculture under the NRICGP program (CSREES‐NRI grant # 2004‐35503‐14116). Financial support in the form of scholarships to AK was provided by AACC International, St. Paul, MN and the Department of Food Science & Human Nutrition, University of Illinois at Urbana‐Champaign. The University of Illinois Research Board provided an equipment research grant.