Comparison of Spectrodensitograms of the Selected Drugs on Different Chromatographic Sorbents

Abstract

The spectrodensitograms of α‐tocopherol acetate, α‐tocopherol, cholecalciferol, estradiol, testosterone, and hydrocortisone on different chromatographic sorbents (silica gel 60, silica gel 60F₂₅₄, mixture of silica gel 60 and kieselguhr F₂₅₄, neutral aluminium oxide 60F₂₅₄, neutral aluminium oxide 150F₂₅₄, and RP18W) were compared. The resultant spectrodensitograms of the studied compounds indicate that applied sorbents have an influence on the wavelength of the obtained fundamental absorption band (λ_max) and the additional absorption bands, as well as on their intensity values [AU]. This fact indicates the need for standaridization of the spectrodensitometric investigations regarding the applied chromatographic conditions.

Keywords:

• TLC
• Spectrodensitometry
• Lipophilic vitamins
• Steroid hormones
• Chromatographic sorbents

Acknowledgment

This research was financed by the Ministry of Science and Information Society Technologies by resources reserved for science in the years 2005–2008 as research project No. 3 T09A 155 29.