Separation of Hexabenzyldigermoxane and Hexabenzyldigermanium by HPLC

Abstract

In this paper optimal conditions of chromatographic determination and separation of hexabenzyldigermoxane and hexabenzyldigermanium by the HPLC method are presented. These compounds cause numerous difficulties in chromatographic processes, because they can be retarded on chromatographic columns and are characterized by long retention times. In our studies, types of stationary phases as well as types of mobile phases and contents were taken into consideration. Obtained results showed unambiguously, that the most selected is the aryl stationary phase RP Si‐NAF. It exhibited the highest separation factor (α), i.e., 1.11 for mobile phase acetonitrile/water. However, considered as the standard (reference) phase, RP Si‐C₁₈ exhibited the maximum separation factor (α), only 1.03 for the solvent system methanol/water. Besides, when the octadecyl column was used, the retention time was three times longer, compared with the use of the analogous aryl column.

Keywords:

• Stationary phases
• Aryl
• Octyl
• Octadecyl
• Benzyl
• Germanium derivatives
• π‐π Interactions
• Chromatography

Acknowledgment

We would like to thank Mrs. Iwona Uszyńska for her help with synthesis — grateful authors.