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Abstract
Identification of complex acyl lipids ideally includes the normal‐phase HPLC to separate the acyl lipid classes followed by reversed‐phase HPLC to separate the molecular species of a lipid class. Both polar lipid classes and non‐polar lipid classes have been separated by normal‐phase HPLC, mostly on silica and diol columns using binary gradients. The tentative identification of lipid classes can be made by HPLC retention times and the co‐chromatography with lipid class standards. Various normal‐phase HPLC systems separating lipid classes are reviewed here.