Thin Layer Chromatographic Techniques (TLC, OP TLC) for Determination of Biological Activated Compounds from Herb Extracts

Abstract

In the current contribution the comparison of various chromatographic techniques (TLC, OP TLC) for the determination of volatile organic compounds including monoterpenes from peppermint (Folium Mentha piperita) is presented. Flavonoids from hawthorn (Crategus oxyacantha), Passiflora incarnata, hop (Humulus lupulus), cacao (Theobroma cacao), as well as tea (Thea sinensis) extracts were also determined by TLC and OP TLC. Planar chromatographic techniques, thin‐layer chromatography (TLC) and over pressure thin‐layer chromatography (OP TLC) have been developed for the quantitative determination of constituent menthol and menthone in extracts of peppermint leaves. The optimisation of separation of monoterpenes from peppermint based on their principles of polarity including the selection of solvents, stationary phases, and chromatographic conditions, was performed. For separation of determined compounds from plant material, different extraction methods such as supercritical fluid extraction (SFE) and solvent extraction were used.

Keywords:

• Sample preparation
• TLC
• OP TLC
• Herbs analysis