Digitized Chromatographic Fingerprint Spectrum of Water Soluble Components of Salvia Miltiorrhiza Bunge by High Speed Countercurrent Chromatography

Abstract

Water soluble components are a major part of active components of Salvia miltiorrhiza Bunge, a very important species of TCM, whose quality control is valuable. Twelve water extracted crude samples of Salvia miltiorrhiza Bunge were analyzed and fingerprints of them were developed by high speed countercurrent chromatography (HSCCC). Three major components in each sample were identified. Their digitized chromatographic fingerprint spectrum (DCFS) were developed by an improved method with relative retention time, relative peak area, relative standard deviation of relative retention time, relative standard deviation of relative peak area, difference rate, and fingerprint characteristic. HSCCC‐DCFS was a quantitative and intuitive method for the fingerprinting of water soluble components of Salvia miltiorrhiza Bunge.

Keywords:

• High speed countercurrent chromatography (HSCCC)
• Digitized chromatographic fingerprint spectrum (DCFS)
• 3,4‐Dihydroxyphenyllactic acid
• Salvianolic acid B
• Protocatechualdehyde
• Salvia miltiorrhiza Bunge

Acknowledgments

This work was supported by National Natural Science Foundation Committee of China (20606032 and 20636010).