High Performance Liquid Chromatography in Analysis of Compounds Comprising the Elements of Bacterial Peptidoglycan Structure with Immunological Activity

Abstract

Numerous non‐toxic peptidoglycan fragments of low molecular weight represent an important group of immunomodulators, and antitumor and antiviral drugs. They were isolated from natural sources or prepared by chemical synthesis. N‐acetylmuramyl‐L‐alanyl‐D‐isoglutamine (muramyl dipeptide, MDP) was recognized as the minimal structure responsible for the biological activity. Numerous derivatives and analogues of MDP have been synthesized by modifications of the muramyl moiety or by changing the composition and configuration of the peptide portion. The promising analogs among them are murabutide, muroctasyn, adamantylpeptides, PGM, and derivatives. Isolation, purification, and characterization of peptidoglycan fragments and novel synthesized compounds were performed using the RP‐HPLC. In this article, a short review of HPL‐Chromatographic analyses of representative compounds comprising elements of peptidoglycan structure is given.

Keywords:

• HPLC
• Bacterial peptidoglycan
• Immunological activity

Acknowledgment

The authors thank the Ministry of Science, Education and Sport of Croatia for its financial support of this work (project No. 0021‐002 and 021‐0212432‐2431).

The authors also thank the publishers for the permissions to reproduce the chromatograms from their respective cited publications.