Use of Hydride‐Based Separation Materials for Organic Normal Phase Chromatography

Abstract

The chromatographic properties of two hydride‐based stationary phases (a material with a hydride surface and one with a hydride surface modified by the addition of C₁₈) are tested with several types of compounds that are typically retained in the organic normal phase (ONP) mode. Both phases display ONP retention, but to differing degrees. Various mobile phases are investigated, as well as some gradient elution profiles. Efficiency, peak shape, and reproducibility for the several solutes in both isocratic and gradient elution, as well as the effects of water in the mobile phase, are assessed.

Keywords:

• Hydride
• Normal phase
• Chromatography
• Separation materials

Acknowledgments

The financial support of the National Institutes of Health (Grant GM 0063519–01) and the National Science Foundation (CHE 0094983) is gratefully acknowledged. JJP would like to acknowledge the support of the Camille and Henry Dreyfus Foundation through a Scholar Award. Symyx Technologies is gratefully acknowledged for allowing the use of its facility and chemicals for this research work.