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Journal of Liquid Chromatography & Related Technologies Volume 31, 2007 - Issue 2
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Original Articles

Simultaneous HPLC Determination of Levamisole Hydrochloride and Anhydrous Niclosamide in Veterinary Powders, and its Validation

Siti Cholifah Analytical Development Section, Department of R & D, Bernofarm Pharmaceutical Company, Surabaya, Indonesia
,
Wiwin Farina Kartinasari Analytical Development Section, Department of R & D, Bernofarm Pharmaceutical Company, Surabaya, Indonesia
&
Gunawan Indrayanto Assessment Service Unit, Faculty of Pharmacy, Airlangga University, Surabaya, IndonesiaCorrespondence[email protected]
Pages 281-291 | Received 17 Jun 2007, Accepted 11 Jul 2007, Published online: 14 Dec 2007
 
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Abstract

A simple, rapid, and validated HPLC method has been developed for simultaneous determination of levamisole hydrochloride and anhydrous niclosamide. A Luna® C‐18 column was used with a mobile phase consisting of acetonitrile–buffer solutions (2∶8, v/v). Quantitative evaluation was performed at 240 nm. The HPLC method is selective, precise, and accurate and can be used for routine analysis of the preparations in pharmaceutical industry quality control laboratories.

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