Preparative Isolation of Kaempferol Pyranosides from a Traditional Chinese Herb using High Speed Countercurrent Chromatography

Abstract

Preparative countercurrent chromatography has been used successfully for the isolation and purification of kaempferol pyranosides including kaempferol‐3‐O‐β‐D‐glucopyranosyl (2‐1)‐a‐L‐rhamnopyranoside, kaempferol‐3‐O‐β‐D‐glucopyranoside, and kaempferol‐3‐O‐a‐L‐rhamnopyranoside from the traditional Chinese herb “Jin qian cao”‐Lysimachia christinae Hance with a two‐phase solvent system composed of ethyl acetate‐methanol‐water with a volume ratio of 50∶1∶50, v/v. The composition of the phase system was optimized using analytical high speed countercurrent chromatography (HSCCC). The crude extract in the amount of 924 mg was separated, yielding 69.8 mg of kaempferol‐3‐O‐β‐D‐glucopyranosyl (2‐1)‐a‐L‐rhamnopyranoside, 45.3 mg of kaempferol‐3‐O‐β‐D‐glucopyranoside, both at a high purity of over 97% and 8.9 mg of kaempferol‐3‐O‐a‐L‐rhamnopyranoside with the purity of 92%.

Keywords:

• High speed countercurrent chromatography
• Kaempferol‐3‐O‐β‐D‐glucopyranoside
• Kaempferol‐3‐O‐a‐L‐rhamnopyranoside
• Kaempferol‐3‐O‐β‐D‐glucopyranosyl (2‐1)‐a‐L‐rhamnopyranoside
• Lysimachia christinae Hance

Acknowledgments

Financial support from Beijing Commission of Science & Technology is gratefully acknowledged.