264
Views
10
CrossRef citations to date
0
Altmetric
Original Articles

Comparison of Extraction and HPLC Methods for Marine Sedimentary Chloropigment Determinations

, &
Pages 1162-1180 | Received 03 Oct 2007, Accepted 19 Nov 2007, Published online: 27 Mar 2008
 

Abstract

This study compared different extraction and high performance liquid chromatography (HPLC) combinations for the analysis of chloropigments in sediments and microalgae (phytoplankton). A significant literature review is also included. Extractants in the present study included 100% acetone, tetrahydrofuran, and an aqueous mixture of methanol, acetone, and dimethylformamide. HPLC methods involved gradient elution methods with or without ion pairing reagents over C18 silica based columns. Though slight differences were found, most notably with the extraction of steryl chlorin esters, this intercomparison reveals that both extraction and HPLC protocols investigated, in any combination, perform well for the analysis of chlorophylls and their derivatives in sediment and phytoplankton samples.

Acknowledgments

This work was done in the framework of the grant No. N00014‐04‐1‐404 of the Department of the U.S. Navy: Naval Research International Field Office (M. S.‐Z.), as well as contract #ML061237 from the South Florida Water Management District (J.W.L.).

Log in via your institution

Log in to Taylor & Francis Online

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 61.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 583.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.