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Abstract
A liquid chromatographic method is described for the determination of taurine‐conjugated cholic, deoxycholic, chenodeoxycholic, and lithocholic acids in dog serum. Samples are mixed with aqueous formic acid, cleaned‐up by solid phase extraction, and analyzed by liquid chromatography with detection at 195 nm. Using a mobile phase of acetonitrile/trifluoroacetic acid solution, the analytes are eluted from the column well resolved from serum interferences. The method shows analytical characteristics well within acceptable limits. Recoveries were in the range 96.2–100.2%, precision, expressed as percent coefficient of variation, in the range 1.8–9.5%, and limits of detection in the range 0.04–0.1 µg/mL.