Chromatography of Biologically Active Chlorides of (E)-N-o-(m- or p-)chlorobenzyl-γ-azastilbenols-2′(3′ or 4′)

Abstract

results of investigations concerning optimization of chromatographic separation and determination of (E)-N-o-(m- or p-)chlorobenzyl-γ-azastilbenols-2′(3′ or 4′) are presented. Analyzed isomers show antimicrobial activity. In the investigation, were considered three stationary phases (octadecyl, octyl, and naphthylpropyl), two mobile phases (acetonitrile, dichloromethane), and various intensities of flow. The best selectivity (α₁ = 1.34, α₂ = 1.47) was obtained using naphthylpropyl column and 100% acetonitrile, as the mobile phase. An application of octadecyl phase, recommended by numerous analysts as standard, did not yield satisfactory results.

Keywords:

• (E)-Azastilbenoles
• Isomers
• Stationary phases: Aryl
• Octyl and Octadecyl
• HPLC

Notes

1-Staphylococcus aureus 209P FDA, 2-Streptococcus faecalis ATCC 8040, 3-Bacillus subtilis ATCC 1633, 4-Escherichia coli PZHO 26B6, 5-Klebsiella pneumoniae 231, 6-Pseudomonas aeruginosa 5 R1, 7-Candida albicans PCM 1409 PZH, 8-Microsporum gypseum K_1, 9-Aspergillus fumigatus C1.

^a In the Table are reported only optimum data of analyzed isomers separation.