Simultaneous Determination of Alkaloids and Flavonoids in HMC05 Preparation by HPLC-DAD

Abstract

High performance liquid chromatography with diode array detector (HPLC-DAD) was developed for simultaneous determination of hesperidin, coptisine, palmatine, and berberine in HMC05, a standardized extract of eight different herbs. This method was validated in terms of specificity, linearity (r² > 0.9995), precision (<5.0% RSD), and recoveries (96.6–110.5%). The LOD of these compounds were ranged from 101.6 to 171.7 ng. In addition, these four compounds in HMC05 were identified or tentatively characterized using HPLC-DAD-electrospray mass spectrometry (HPLC-DAD-ESI-MS).

Keywords:

• HMC05
• HPLC-DAD
• Simultaneous determination
• Validation

ACKNOWLEDGMENTS

This study was supported by grants of the Korea Health 21 R & D Project, Ministry of Health & Welfare, Republic of Korea (B050042AM081505N100020B).

Notes

^a y = peak area, x = amount (µg).

^a RSD (%) = (SD of amount detected/mean of amount detected) × 100 (n = 3).