Determination of Biogenic Amines and Vitamins in Urine Samples with HPLC

Abstract

An HPLC system for simultaneous separation and marking of biogenic amines and their metabolites from catecholamines group: dopamine (DA), epinephrine (E), normetanephrine (NMN), metanephrine (MN), 3,4-dihydroxyphenylacetic acid (DOMA), 3-metoxy-4-hydroxyphenyl-glycol (MHPG), homovanillic acid (HVA) and indoloamines group: serotonin (5HT) and 5-hydroxyindole-3-acetic acid (5HIAA), as well as water soluble vitamins B-1, B-2, B-3, B-6, B-12, and C has been developed. The RP18e column with RP18 precolumn and DAD serial connected with a fluorescence detector have been used. Gradient elution with acetate buffer (pH = 4.66) with methanol has been applied. The developed system has been used for marking the examined compounds in urine samples.

Keywords:

• Biogenic amines
• HPLC
• Urine
• Water soluble vitamins

ACKNOWLEDGMENTS

This work was supported from science funds for scientific research for years 2007–2009, as Project No. N20402532/0738.

Notes

nf – not found.