Application of Preparative High-Speed Countercurrent Chromatography for Separation of Elatine from Delphinium shawurense

Abstract

Preparative separation of elatine in Delphinium shawurense was achieved for the first time using high speed countercurrent chromatography (HSCCC). The separation was performed with a solvent system composed of ethyl acetate-chloroform-methanol-water (3:0.1:2:3, v/v) using the lower organic phase as a mobile phase under a revolution speed of 800 rpm. This yielded 72 mg of elatine at over 97% purity with an approximately 95% recovery. The chemical structure was identified by MS and NMR.

Keywords:

• Delphinium Shawurense
• Elatine
• High speed countercurrent chromatography (HSCCC)

ACKNOWLEDGMENTS

This work was financially supported by grant (code: KGC X2-SW-213–08) from Key Project of Knowledge Innovation Program of Chinese Academy of Sciences and grant (code: 200515123) from the program for development and research of high technologies in Xinjiang.