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Journal of Liquid Chromatography & Related Technologies Volume 31, 2008 - Issue 20
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Original Articles

Development of a Liquid Chromatography-Negative ESI-Tandem Mass Spectrometry Method for Ibuprofen with Minimization of Matrix Effects Associated with Phospholipids

Omnia Ismaiel Virginia Commonwealth University, School of Pharmacy, Department of Pharmaceutics, Richmond, Virginia, USA
,
Matthew S. Halquist Virginia Commonwealth University, School of Pharmacy, Department of Pharmaceutics, Richmond, Virginia, USA
,
Magda Y. El-Mammli Zagazig University, Faculty of Pharmacy, Department of Analytical Chemistry, Egypt
,
Abdalla Shalaby Zagazig University, Faculty of Pharmacy, Department of Analytical Chemistry, Egypt
&
H. Thomas Karnes Virginia Commonwealth University, School of Pharmacy, Department of Pharmaceutics, Richmond, Virginia, USACorrespondence[email protected]
Pages 3194-3208 | Received 01 Apr 2008, Accepted 11 Jun 2008, Published online: 22 Oct 2008
 
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Abstract

Phospholipids are known to cause matrix ionization effects during LC/MS/MS analysis of biological samples. Matrix effects involving endogenous phospholipids in the negative ESI mode have been investigated in this work for the determination of ibuprofen in human plasma. Glycerophosphocholines (GPChos) and 2-lysoglycerophosphocholines (2-lyso GPChos) were monitored as markers for endogenous matrix components. Various extraction solvents were evaluated to assess their abilities to remove phospholipids from plasma samples to minimize matrix effects. Post-column infusion experiments were applied to chromatographically resolve matrix effects that resulted from endogenous phospholipids. The resulting method was validated and linearity was obtained over a concentration range of 50 to 10,000 ng/mL.

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