Abstract
Picrasma crenata is a Brazilian tree, which is utilized to treat Diabetes mellitus, gastric perturbation, and hypertension. Quassinoids are the main constituents obtained from the wood of this plant. The relationship between the quassinoids and the biological effects reveal the interest of quantifying these constituents in P. crenata wood, as well as of the validation of the analytical methodologies. This work was designed, therefore, to develop an HPLC system to separate quassin and quantify it in extractive solution from P. crenata. The main validation parameters of the method are also determined. The method showed linearity for quassin in the range 13.13–100 µg/mL. The aqueous extractive solution showed linear response in the range 0.978–6.520 mg/mL. Precision and accuracy were determined for the extractive solution in the concentration of 6.52 mg/mL. The HPLC method showed an excellent performance in separating the quassinoids in P. crenata extracts, since the presence of interference has been previously evaluated.
ACKNOWLEDGMENT
The authors thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for financial support.