Retention Factor in Micellar Liquid Chromatography on the Basis of Linear Solvation Energy Relationships

Abstract

Linear solvation energy relationships (LSERs) are used to investigate the fundamental chemical interactions governing on a C₁₈ column. The micellar liquid chromatographic (MLC) systems using sodium lauryl sulfate (SDS) and a mixture of water with (methanol, n-propanol, and n-butyl alcohol) modifiers were characterized using the LSER model. The ability of the LSERs to account for the chemical interactions underlying solute retention is shown and the effects of the surfactant and modifier concentration on the retention in MLC were discussed. A comparison of predicted and experimental retention factors suggests that LSER formalism is able to reproduce, adequately, the experimental retention factors of the solutes studied in the different experimental conditions investigated. This model is a helpful tool to understand the solute-surfactant interactions and evaluate the retention characteristics of micellar liquid chromatography.

Keywords:

• Linear solvation energy relationships (LSER)
• Micellar liquid chromatography
• Modifier
• Retention
• Surfactant

ACKNOWLEDGMENT

The authors are grateful for financial support from the Center for Advanced Bioseparation Technology, Inha University.