Extraction and Analysis of Liquiritin from Licorice

Abstract

The extraction conditions of liquiritin from licorice were investigated. By changing the different extraction solvents, methods, and extraction times, the optimum extraction condition was methanol as an extraction solvent, and 120 min dipping time under room temperature. The extracts of licorice were separated and determined by reversed-phase high performance liquid chromatography with an acetonitrile/water (20:80, v/v, containing 1% acetic acid) as the mobile phase. Under the optimum extraction condition, 0.085 mg/g of liquiritin was extracted from Chinese licorice and the recovery was around 81.04%.

Keywords:

• Extraction
• Glycyrrhizic acid
• HPLC
• Licorice
• Liquiritin

ACKNOWLEDGMENT

The authors gratefully acknowledge financial support by the Center for Advanced Bioseparation Technology and Inha University, Korea.

Notes

∗not detected.