Preparative Separation of Axifolin-3-Glucoside, Hyperoside and Amygdalin from Plant Extracts by High Speed Countercurrent Chromatography

Abstract

High speed countercurrent chromatography (HSCCC) was successfully used to isolate three bioactive compounds, i.e., amygdalin from bitter almond and taxifolin-3-glucoside and quercetin-3-galactoside (hyperoside) from water extract of A. pilosa Ledeb, respectively. From 1 g of the crude extract 65 mg of amygdalin was isolated at 97% purity using a two phase solvent system composed of ethyl acetate-n-butanol-water (5:2:5, v/v) by preparative HSCCC. From a 400 mg amount of crude extract of A. pilosa Ledeb, 11 mg of taxifolin-3-glucoside and 8 mg of hyperoside were isolated at 96% purity, using a two phase solvent system composed of ethyl acetate-methanol-water (25:1:25, v/v) similarly by preparative HSCCC. The final structural identification was performed by MS, ¹H-NMR, and ¹³C-NMR spectra.

Keywords:

• Agrimonia pilosa Ledeb
• Amygdalin
• High speed countercurrent chromatography (HSCCC)
• Hyperoside
• Taxifolin-3-glucoside

ACKNOWLEDGMENT

Financial support (Project No. 200803022) from Ministry of Agriculture of the People's Republic of China is gratefully acknowledged.