Molecularly Imprinted Solid-Phase Extraction of 1-Methyl-2-piperidinoethylesters of Alkoxyphenylcarbamic Acid from Human Plasma, Comparison with Classical Solid-Phase Extraction

Abstract

Molecularly imprinted polymers for 1-methyl-2-piperidinoethylester of 2-methoxyphenylcarbamic acid (template) have been synthesised using a non-covalent imprinting approach. Acrylamid and methacrylic acid, respectively, were used as the functional monomers. Imprinted polymers were used as the sorbents for solid-phase extraction (MISPE). The capacity and selectivity of prepared imprinted polymers was investigated. The non-imprinted (blank) polymers were prepared by the same way without template to study the non-specific interactions. Molecularly imprinted polymer prepared from methacrylic acid was used to preconcentrate 1-methyl-2-piperidinoethylester of 2-methoxyphenylcarbamic acid and its analogues from spiked human plasma.

Keywords:

• HPLC
• Molecularly imprinted polymer
• SPE

ACKNOWLEDGMENT

Support from the Grant agency Slovak Republic (grants 1/0058/08), APVV project No. 20-035-205 and VEGA project 1/4291/07 is gratefully acknowledged.

Notes

∗The specific binding capacity was calculated by the deduction of the amount non-specifically adsorbed on NIP from the amount adsorbed on MIP.

∗The specific binding capacity was calculated by the deduction of the amount non-specifically adsorbed on NIP from the amount adsorbed on MIP.

∗Coelution with another compound from plasma.