Abstract
In this paper, a novel HPLC method was developed for analyzing imperatorin and isoimperatorin in the SFE extracts of Angelica dahurica. The separations were effected by using a mobile phase of methanol and water. The gradient elution of the mobile phase was 80% in 0–5 min, 80–68% in 5–7 min, and 68% in 7–12 min. The detector wavelength was set at 249 nm. Analytical characteristics of the separation, such as limit of detection, limit of quantification, linear range, and reproducibility were evaluated. The developed method was applied to optimizing supercritical fluid extraction of coumarins from Angelica dahurica. The maximum extraction yield was obtained at 30 MPa and 50°C with a flow rate of 25 L/h and extraction time of 2 h.
ACKNOWLEDGMENTS
This study was supported by the key grants from the Science and Technology Department of Zhejiang Province, China (Grant No. 2006C13011 and No. 2007C23023).
Notes
Note. a y: peak area; x: concentration (µg/mL).