Determination of Novel Plant Growth Promoting Diterpenes in Callicarpa macrophylla by HPLC and HPTLC

Abstract

Methods based on HPTLC and RP-HPLC with UV detection for rapid quantitative determination of two major plant growth promoters in Callicarpa macrophylla, calliterpenone (1) and calliterpenone monoacetate (2) are described. The recoveries of the two compounds were between 97.5–100.8% by HPTLC method and 99.3–100.9% by HPLC assay. The relative standard deviations of the two compounds ranged between 1.26–1.68 (Intra-day) and 1.06–1.68 (Inter-day) for HPTLC and 0.02–0.92 (Intra-day) and 0.03–0.92 (Inter-day) for HPLC. The methods were used for routine analysis of two compounds in the leaves of the plant.

Keywords:

• Callicarpa macrophylla
• Calliterpenone, Calliterpenone mono acetate
• diterpenes
• Method comparison

ACKNOWLEDGMENTS

The authors wish to thank Council of Scientific and Industrial Research (CSIR), New Delhi for financial supports in the form of major laboratory project. The authors are grateful to the Director, CIMAP, Lucknow for providing necessary facilities during the work.

Notes

^a Correlation of spectrum at start of peak with spectrum at the centre of peak at 610 nm scanning.

^b Correlation of spectrum at center of peak with spectrum at the end of peak at 610 nm scanning.

^a Retention time in HPLC analysis.

^b Retention factor in HPTLC analysis.