DETERMINATION OF NEW GENERATION PESTICIDES IN COMPLEX PLANT MATRICES BY HPLC USING VARIOUS STATIONARY PHASES

Abstract

Author interest is focused on method optimization to determine the new compounds using stationary and mobile phases with different physicochemical properties. This work deals with 5 substances composed of nitrogen containing heterocycles, 1,2,4-triazoles. The relationships between the chromatographic retention factor (k) and those physicochemical properties which are relevant in quantitative structure retention relationship (QSRR) studies were investigated. The accuracy of the simple linear regressions between the chromatographic retention and the descriptors for all of the compounds was satisfactory (correlation coefficient, 0.8 ≤ r² ≤ 1.0). The QSRR models of compounds could be predicted with a multiple linear regression equation having the statistical index, r² = 1.000. Evaluation of chromatographic properties of the new stationary phases and description of the molecular separation mechanism using the QSRR method including molecular modeling will be suggested.

Keywords:

• HPLC columns
• QSRR
• retention factor
• triazole fungicides

ACKNOWLEDGMENTS

I thank Prof. Bogusław Buszewski (Nicolaus Copernicus University, Toruń, Poland) for valuable discussions and suggestions.

This work was supported by Ministry of Science and Higher Education during 2005–2008, grant No. 2P06 R 075 29 (Warsaw, Poland).

Notes

I.D. – column ID.

d_p – particle size.

P_c – carbon load.

S_BET – specific surface area.

D – pore size.

EC – end-capping.