ISOLATION AND PURIFICATION OF PHENOLIC COMPOUNDS FROM MAGNOLIAE OFFICINALIS BY PREPARATIVE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract

A rapid, facile, and economical method to isolate and purify phenolic compounds (honokiol, magnolol, and 4-O-methylhonokiol) from the Chinese traditional herb Magnoliae officinalis was described. The isolation of honokiol, magnolol, and 4-O-methylhonokiol were performed by preparative high performance liquid chromatography (HPLC) on a C₁₈ (300 × 50 mm, 10 µm) column using mobile phase methanol and 1% (v/v) acetic acid in water (85/15, v/v), at a flow rate of 85 mL/min and with a detection wavelength of 294 nm. The purified compounds were characterized by ¹H and ¹³C NMR. The technique described in this article is a fast, economical, and highly efficient tool in the large scale isolation of phenolic compounds.

Keywords:

• honokiol
• HPLC
• Magnoliae officinalis
• magnolol
• 4-O-methylhonokiol
• preparative HPLC
• purification

Notes

Note: a is honokiol; b is magonol; c is unknown compound; t_R is retention time(min); k′ is capacity factor; α is separation factor; Rs is resolution; Rs₁ is the resolution of a with b; Rs₂ is the resolution between b and c.