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Abstract
Today, chromatography is the back bone of separation science and is being used in all research laboratories and industries of the world. It has developed into nano liquid chromatography (NLC) modality through various kinds of chromatography. The present article describes a journey from Mikhail Tswett in 1903 to today of NLC. Attempts have been made to discuss the applications of newly developed NLC, especially for proteomic and genomic researches. Few chromatograms of NLC have been given for ready reference. The chromatographic journey is shown in the form of a chromatography tree. NLC is eco-friendly and may be referred to as green or clean chromatography. The future perspectives of chromatography have been highlighted.