SEPARATION AND DETERMINATION OF ALKYLFURYLSILANE ISOMERS FOR HPLC

Abstract

In this paper are presented optimal conditions of chromatographic separation of two isomers: γ-(chloropropyl)-3-furyldimethylsilane and [2-chloro(1-methyl)ethyl](3′-furyl)dimethylsilane. These isomers show the ability of modification of the surface of silica gel. In the investigation were tested three stationary phases and three mobile phases: dichloromethane, methanol, and a mixture of dichloromethane and hexane (80/20, v/v), in different flow intensities. An application of octadecyl stationary phase, which is recommended as a standard phase, did not yield separation of the isomers, independently of composition and flow of a mobile phase. Better results, but with asymmetric peaks have been obtained on the octyl phase. Finally, aryl chemically bonded phases permitted acquiring good separation of the isomers in a time shorter than 25 min. The best separation factor (1.11) has been obtained on the chemically bonded aryl phase using dichloromethane with flow of 0.1 mL · min⁻¹. Therefore, the best selectivity in separation of above mentioned compounds exhibited the aryl chemically bonded phase.

Keywords:

• aryl
• 3-furylsilanes
• isomers
• octadecyl
• octyl
• stationary phases

Notes

∗In the table are presented only these data of isomer separation, which are concerning retention time shorter than 50 min.