CENTRIFUGAL PARTITION CHROMATOGRAPHY: APPLICATION TO NATURAL PRODUCTS IN 1994–2009

Abstract

Centrifugal partition chromatography (CPC) is a type of counter current separation technique and was first invented by Sanki Eng. Ltd. in Kyoto. Since the year of 1982, CPC has been used to isolate or fractionate bioactive materials and has shown its efficiency in the purification process of natural products from plants and microorganisms. This review focuses on the CPC applications for the purification of over 250 compounds from 60 plant materials and microorganisms between 1994 to September 2009.

Keywords:

• centrifugal partition chromatography
• CPC application
• microorganism
• natural product
• plant

Notes

^a BuOH: n-butanol; CHCl₃: chloroform; CyHex: cyclohexane; EtOAc: ethyl acetate; H₂O: water; Hep: n-heptane; Hex: n-hexane; IPA: isopropyl alcohol; Me₂CO: acetone; MeCN: acetonitrile; MeOH: methanol; MtBE: methyl tert-butyl ether; pet-ether: petroleum ether; TFA: trifluoroacetic acid; Tol: toluene; LP: lower phase; UP: upper phase; SP: stationary phase; MP: mobile phase.

^b CPC was used for separation (S) or fractionation (F).

^a EtOAc: ethyl acetate; H₂O: water; IPA: Isopropyl alcohol.

^b CPC was used for separation (S) or fractionation (F).

^a EtOAc: ethylacetate; Hex: n-hexane; H₂O: water; MeOH: methanol.

^b CPC was used for separation (S) or fractionation (F).

^a BuOH: n-butanol; CHCl₃: chloroform; CH₂Cl₂: methylene chloride; H₂O: water; Hex: n-hexane; IPA: Isopropyl alcohol; MeOH: methanol.

^b CPC was used for separation (S) or fractionation (F).

^a EtOAc: ethyl acetate; H₂O: water; Hep: n-heptane; Hex: n-hexane; MeOH: methanol.

^b CPC was used for separation (S) or fractionation (F).

^a BuOH: n-butanol; CHCl₃: chloroform; EtOAc: ethyl acetate; H₂O: water; Hep: n-heptane; Hex: n-hexane; MeOH: methanol; HCOOH: formic acid; IPA: isopropyl alcohol; LP: lower phase; UP: upper phase; SP: stationary phase; MP: mobile phase.

^b CPC was used for separation (S) or fractionation (F).

^a AcOH: acetic acid; BuOH: n-butanol; CHCl₃: chloroform; CH₂Cl₂: methylene chloride; C₆H₆: benzene; EtOAc: ethyl acetate; H₂O: water; HBr: hydrogen bromide; Hep: n-heptane; Hex: n-hexane; MeCN: acetonitrile; MeOH: methanol; MtBE: methyl tert-butyl ether; PrOH: propanol; TEA: triethylamine;.

^b CPC was used for separation (S) or fractionation (F).

^a BuOH: n-butanol; CHCl₃: chloroform; EtOH: ethanol; H₂O: water; Hex: n-hexane; MeCN: acetonitrile; MeOH: methanol; K₂HPO₄: dipotassium phosphate.

^b CPC was used for separation (S) or fractionation (F).

^a BuOH: n-butanol; DEA: diethanoamine; EtOAc: ethyl acetate; H₂O: water; Hex: n-hexane; HCl: hydrogen chloride; H₃PO₄: phosphoric acid; Me₂CO: acetone; MeCN: acetonitrile; MeOH: methanol; TEA: triethanolamine; Tol: toluene.

^b CPC was used for separation (S) or fractionation (F).