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Abstract
In this article, a novel method, microwave assisted micellar extraction coupled with high performance liquid chromatography has been developed for the determination of five anthraquinone derivatives (aloe-emodin, rhein, emodin, chrysophanol, physcion) in semen cassiae. Some important parameters, such as the pH value of the solution, microwave assisted micellar extraction time, extraction power, concentration of surfactant Genapol X-080 solution, liquid to solid ratio, and semen cassiae granularity were investigated. Optimum extraction conditions were obtained as follows: 0.1 g 180 μm particle size of semen cassiae, 10% Genapol X-080 (w/v), pH 3.0, 260 w microwave power, microwave assisted extraction time for 3 min, and liquid/solid ratio of 60:1 (mL/g). The method yields linear calibration curves in the concentration range of 1.0 to 40 μg/mL for target analytes, and the detection limits for the five anthraquinone derivatives were between 6.0 ng/mL and 39 ng/mL. The relative standard deviations (R.S.D.) were less than 1% (n = 6). In addition, the MAME method established in this article is demonstrated to be a simple, economic, and environmentally friendly alternative to conventional organic solvent extraction methods.
ACKNOWLEDGMENTS
Financial support from the National Natural Science Foundation of China (20575016, 20875020) and the Natural Science Foundation of Hebei Province China (B2006000953) are gratefully acknowledged. The authors also thank “the introduction of talents” program of Hebei University (project no. 036) for financial support.
Notes
X denotes concentration (µg/mL) of the Semen Cassiae, Y denotes peak area.