HPLC DETERMINATION OF GUANIDINO COMPOUNDS IN SERUM OF UREMIC PATIENTS USING PYRIDOIN AS DERIVATIZING REAGENT

Abstract

Guanidino compounds: guanidine (GD), methylguanidine (MG), guanidinoacetic acid (GAA), guanidinopropionic acid (GPA), guanidinobutyric acid (GBA), arginine (Arg), guanidinosuccenic acid (GSA), and creatinine (CRN) as derivatives of pyridoin were separated from Kromasil C-18, 5 μm column, when eluted with methanol-water-sodium tetraborate buffer (0.1 M, pH 8.8) (57:28:15 v/v/v) with a flow rate of 1 mL/min. The UV detection was at 228 nm, linear calibrations were observed with 1.14–141 μmol/L and limits of detection (LOD) within 0.039–0.070 μmol/L. The method was used for the determination of guanidino compounds from serum of uremic patients, and the results have been interpreted as compared to that of healthy volunteers. The results obtained were reproducible (n = 4) with RSD within 4.2% and were higher in uremic patients as compared to healthy volunteers.

Keywords:

• guanidino compounds
• healthy volunteers
• HPLC
• pyridoin
• uremic patients