DETERMINATION OF METFORMIN IN CULTIVATED PLANT SPECIES AND SOIL BY LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY

Abstract

A liquid chromatographic-mass spectrometry (LC-MS/MS) method for the determination of metformin in cultivated plant species and soil is described. Plant samples (barley, barley-leaf, carrot, etc.) or soil were homogenized with an extraction solution of ammonium acetate/formic acid and centrifuged. The supernatant was clean-up with a solid phase extraction column, filtered, and injected into the LC/MS-MS. The limits of quantification were 30 ng/g for metformin.

Keywords:

• cereals
• LC-MS/MS
• metformin
• plants
• root vegetables
• seed
• soil

ACKNOWLEDGEMENTS

The authors would like to thank The Research Council of Norway for financial support. Project no. 1848339/I10.

Notes

TE#1 = events.

S.D. = Standard deviation.