ENANTIOSEPARATION USING A CELLULOSE-BASED STATIONARY PHASE BY CAPILLARY LIQUID CHROMATOGRAPHY

Abstract

A 3,5-dimethylphenylcarbamate derivative of cellulose immobilized onto the aminopropyl-modified silica gel was used as a chiral stationary phase and separation of five enantiomers was successfully performed by a home-made chiral capillary liquid chromatography system. Different chromatographic parameters such as resolution, column efficiency, and retention time were comparatively investigated using three chiral stationary phases with different coatings. The key factors which may influence the performance of the chiral stationary phases were evaluated by varying the composition of the mobile phase and its flow rate. It was illustrated that the self-installed chiral separation system was characterized by simplicity, high resolution, ease for practical usage, and could be handily applied for enantioseparation at the conventional laboratory.

Keywords:

• cellulose tris(3,5-dimethylphenylcarbamate)
• capillary column
• capillary liquid chromatography
• chiral stationary phases
• enantiomer separation
• slurry packing

ACKNOWLEDGMENT

The financial support by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry, and the National Natural Science Foundation of China (Nos. 30070506, 30771436) are gratefully acknowledged.

Notes

Mobile phases: (a) HEX/IPA 90:10(v/v), (b) HEX/IPA 70/30(v/v), (c) HEX/IPA 95/5(v/v); Flow rate: 0.5 mL/min, room temperature.