DETERMINATION OF HYDROCORTISONE IN PHARMACEUTICAL DRUG BY TLC WITH DENSITOMETRIC DETECTION IN UV

Abstract

Adsorption thin layer chromatography with densitometry has been established for the identification and the quantification of hydrocortisone in tablets. UV densitometry was performed in absorbance mode at 250 nm. The presented method was validated by specificity, range, linearity, accuracy, precision, detection limit, quantitative limit, and robustness. The TLC-densitometric method was also compared with a pharmacopeil UV-spectrophotometric method for the assay of hydrocortisone, and the results confirmed statistically that the TLC-densitometric method can be used as a substitute method.

Keywords:

• densitometry
• hydrocortisone
• pharmaceutical analysis
• quantitative analysis
• spectrodensitometry
• spectrophotometry
• TLC

ACKNOWLEDGMENT

This research was financed by the Ministry of Science and Higher Education by resources reserved for science in the years 2005–2008 as research project No. 3T09A 155 29 and by the Medical University of Silesia as part of statutory research project KNW-1-001/10.

Notes

^a A – peak area [AU], x – amount [µg spot⁻¹] of drug analyzed, r – correlation coefficient, R – recovery [%], CV – coefficient of variation [%].