Abstract
Simple, rapid, reproducible capillary zone electrophoresis (CZE) methods had been developed for the separation and determination of alkaloids in Rhizoma corydalis and its preparation. Four experimental parameters including electrolyte concentration, pH, organic modifier, and applied voltage were optimized. The optimum condition: 60 mM disodium hydrogen phosphate and 55% (v/v) methanol, 24 kV applied voltage, and temperature 30°C. Under the pH of running buffer (pH 4.27) and detection wavelength 214 nm, tetrahydropalmatine was successfully separated. Under the pH of running buffer (pH 2.80) and detection wavelength 254 nm, palmatine was base line separated. The linearity between peak areas and the concentrations of the analytes were also investigated, and excellent linear behavior was obtained.
ACKNOWLEDGMENTS
This work was supported by the National Natural Science Foundation of China (No. 30772721, 30672600), the Great Research Project of Chinese Academy of Sciences (KGCX2-SW-213-06), the Natural Science Foundation of Jilin Province (No.20060902), and the Natural Science Foundation of Changchun City (04-02GG233).