CHEMOMETRIC CHARACTERIZATION OF MODEL COMPOUNDS RETENTION IN SALTING-OUT THIN LAYER CHROMATOGRAPHY ON SILICA

Abstract

Thirty-five simple model compounds were chromatographed on silica plates with twelve inorganic salts dissolved in high concentrations (0.5–4 mol/L) in water. The resulted retention dataset was analyzed chemometrically by Principal Component Analysis to find interesting dependences and trends in the retention. It can be concluded, that first PC (explaining majority of variance) is connected with compound contribution, independent of the salt used. The differences between the salting-out effect intensities are modeled by second PC. The third PC mainly explains differences in retention between salts, whereas the fourth one represents differences between salting-out intensities among salts.

Keywords:

• chemometrics
• model compounds
• retention
• salting-out thin layer chromatography

ACKNOWLEDGMENT

We acknowledge computational support of Interdisciplinary Centre of Mathematical Modelling, Warsaw University, Poland (Grant No. G36-8).