SIMULTANEOUS DETERMINATION OF PAEONOL AND FERULIC ACID IN SU-XIAO-XIN-TONG DROPPING PILLS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH MATRIX SOLID-PHASE DISPERSION EXTRACTION

Abstract

—Matrix solid-phase dispersion (MSPD) extraction and HPLC have been used for determination of paeonol and ferulic acid in “Su-Xiao-Xin-Tong Dropping Pills” (SXDP). For extraction of these compounds, the type of sorbent, the type and volume of elution solvent were studied. The highest yields were obtained with a ratio 4:1 of C₁₈ to sample and 10 mL methanol-water-glacial acetic acid (80:20:0.5, v/v). The chromatographic separation was achieved with methanol:water:glacial acetic acid (50:50:0.5, v/v) as mobile phase at a flow rate of 1.0 mL/min, a Dikma Diamonsi-C₁₈ column (250 mm × 4.6 mm, 5 µm), and UV detection at 315 nm. Linearity (R² > 0.999), intra- and inter-day (RSD < 2.0%) precision were determined. The linearities of paeonol and ferulic acid were in the range of 25–250 µg/mL and 2.5–25 µg/mL, respectively. The limit of detection was found to be 0.080 µg/mL for paeonol and 0.030 µg/mL for ferulic acid. The mean recoveries for paeonol and ferulic acid were 94.5 and 93.3%, respectively. It is a simple and sensitive analytical method with good accuracy and reproducibility.

Keywords:

• ferulic acid
• HPLC
• method development
• MSPD
• paeonol
• su-xiao-xin-tong dropping pills

ACKNOWLEDGMENTS

This work was financially supported by the Research Project of Administration of Traditional Chinese Medicine of Heilongjiang Province, China (Grant No. ZHY 10-W69).

Notes

^a Mean = Average of three determination; S.D. = Standard deviation. ^b n = Number of determination. ^c RSD ˭ Relative standard deviation.

^a n = Number of determination; ^b k = Asymmetry factor; ^c RSD˭Relative standard deviation.