APPLICATION OF HPLC METHOD FOR INVESTIGATION OF STABILITY OF NEW BENZIMIDAZOLE DERIVATIVES

Abstract

Obtained benzimidazole derivatives, our newly synthesized heterocyclic compounds, belong to a new group of chemical bondings with potential anticancer features. A biochemical study determining anticancer activity of benzimidazole derivatives was conducted in the environment of 0.2% DMSO (dimethyl sulfoxide). The HPLC method was used to determine stability of these compounds in a biochemical study environment. Optimization of the chromatographic system and validation of the established analytical method were performed. Stability analysis of the derivatives was examined in the reversed phases RP-8 system, where pure acetonitrile was used as an eluent for the analysis of compound 1–4. The HPLC analysis revealed that the compounds studied are homogeneous in the environment of 0.2% DMSO up to 96 hr.

Keywords:

• Anti-cancer drugs
• benzimidazole
• HPLC
• hypoxia
• nitobenziyidazole
• RP-8

ACKNOWLEDGMENTS

HPLC analytical research is supported by the Medical University of Łódź, Poland (503-3015-1, Grand No. 507-13-052).

Notes

Time retention (t_R), peak width (w), retention coefficient (k), number of theoretical plates (n).

Arithmetic mean (), standard deviation (s), mean ± SD (µ), relative standard deviation [%] (RSD).

Standard deviation (s), mean ± SD (µ), relative standard deviation [%] (RSD).

Arithmetic mean .