COMPARISON OF NP-TLC AND RP-TLC WITH DENSITOMETRY TO QUANTITATIVE ANALYSIS OF TOCOPHEROL ACETATE IN PHARMACEUTICAL PREPARATION

Abstract

NP-TLC and RP-TLC techniques with the densitometry were compared to the determination of tocopherol acetate in oral fluid Vitaminum E. It was stated, that tocopherol acetate was better separated from tocopherol using NP-TLC technique than RP-TLC technique. Therefore, NP-TLC with densitometry has been used for the identification and the quantitative determination of tocopherol acetate in pharmaceutical preparation. The NP-TLC method with densitometry was validated. The accuracy of the method was evaluated by measurement of recovery. According to the results of the experiments performed using the NP-TLC-densitometric method, it was determined that the procedure used in this study is reliable with specificity, accuracy, precision, and robustness. It could be said that the NP-TLC-densitometric method is suitable for the routine analysis of tocopherol acetate in quality control laboratories.

Keywords:

• densitometry
• NP-TLC
• pharmaceutical analysis
• quantitative analysis
• RP-TLC
• spectrodensitometry
• tocopherol acetate

ACKNOWLEDGMENTS

This research was financed by the Medical University of Silesia as part of statutory research projects KNW-1-001/10 and KNW-1-004/P/1/0.

Notes

^a A, peak area [AU]; x, amount [µg spot⁻¹] of drug analyzed; r, correlation coefficient; R – recovery [%]; CV, coefficient of variation [%].