DEVELOPMENT AND VALIDATION OF HPTLC METHOD TO DETECT CURCUMIN AND GALLIC ACID IN POLYHERBAL FORMULATION

Abstract

Curcumin and Gallic acid are one of the phytoconstituents present in Curcuma longa and Emblica officinalis, respectively. Both these plants are used traditionally in the treatment of cancer and for the treatment of leukemia. In the present study, an attempt has been made to develop a HPTLC method for quantitative estimation of curcumin and gallic acid in different anticancer polyherbal formulations. The method employed TLC aluminum plates precoated with silica gel 60 F₂₅₄ as the stationary phase. The solvent system consisted of chloroform:ethyl acetate:formic acid (7.5 mL + 6 mL + 0.5 mL). This system was found to give compact spots for curcumin (Rf value of 0.55 ± 0.02) and for gallic acid (Rf value of 0.26 ± 0.03). Densitometric analysis of curcumin and gallic acid were carried out in the absorbance mode at 254 nm. This HPTLC method was found to be reproducible, accurate, and can detect curcumin and gallic acid at a nanogram level. The developed HPTLC method would be an important tool in the quality control method for polyherbal formulations.

Keywords:

• curcuma longa
• emblica officinalis
• HPTLC
• polyherbal formulation
• standardization
• validation