A VALIDATED LC-MS METHOD FOR THE DETERMINATION OF BACITRACIN DRUG RESIDUES IN EDIBLE PORK TISSUES WITH CONFIRMATION BY LC-TANDEM MASS SPECTROMETRY

Abstract

In this study, liquid chromatography–mass spectrometry was used to quantify the concentrations of residues of bacitracin A in extracts obtained from pork liver, kidney, and muscle tissues. Tissues were homogenized with acidic methanol/water and centrifuged. The supernatant was decanted, diluted with water, and loaded onto a conditioned solid phase extraction (SPE) cartridge. Bacitracin A was eluted from the SPE with methanol. After evaporating the methanol to dryness, the residual extract was reconstituted in the mobile phase for quantitative analysis by LC-MS. The limit of Quantification (LOQ) for the method was 100 ng/g. Any samples eliciting quantifiable concentrations ≥100 ng/g were subjected to confirmatory analysis by LC-MS/MS.

Keywords:

• bacitracin A residues
• confirmatory analysis
• edible pork tissues
• LC-MS
• LC-MS/MS
• quantitative analysis

Notes

^a Australian Pesticides and Veterinary Medicine Authority at http://www.apvma.gov.au/residues/docs/table01_april_2010.pdf.

^b European Agency for the Evaluation of Medicinal Products at: http://www.ema.europa.eu/pdfs/vet/mrls/076800en.pdf.

^c Laws of Hong Kong Cap 132 AF and 139 N at: http://www.legislation.gov.hk/blis_pdf.nsf/6799165D2FEE3FA94825755E0033E532/3098610F7678AC3F482575EE004458A1/$FILE/CAP_139N_e_b5.pdf.

^d Part 556 Title 21, Volume 1, CFR, Food and Drug Administration at: http://ecfr.gpoaccess.gov/cgi/t/text/text-idx?c=ecfr&sid=536cdf44b4394d11c6bcce0dfbabf21b&rgn=div8&view=text&node=21:6.0.1.1.17.2.1.10&idno=21.

**[BAC] = BAC in square brackets means the concentration of BAC.