DEVELOPMENT AND VALIDATION OF AN ISOCRATIC HPLC METHOD FOR THE SIMULTANEOUS DETERMINATION OF RESIDUAL MONOMERS RELEASED FROM DENTAL POLYMERIC MATERIALS IN ARTIFICIAL SALIVA

Abstract

A simple and rapid method for the simultaneous determination of Bisphenol-A (BPA), Triethylene glycol dimethacrylate (TEGDMA), Bisphenol A glycerolate dimethacrylate (BIS-GMA), and Urethane dimethacrylate (UDMA) from dental polymeric materials in artificial saliva is described. Chromatographic analysis was performed isocratically on a Kromasil 100-C₁₈ analytical column (25 cm × 4.6 mm id, 5 µm) with CH₃CN:H₂O, 75:25%, v/v as mobile phase within 6 min. The developed method was validated in terms of selectivity, linearity, accuracy, precision, and sensitivity. Accuracy and precision were examined at three concentration levels. Repeatability and between-day precision was examined over a period of five days and revealed RSD values lower than 11.2%. The relative errors ranged from −13.8% to 3.9%. In artificial saliva the limit of quantification (LOQ) was calculated as 1.2–3.6 ng/µL. No interference was observed under described experimental conditions. Residual monomers released from dental materials were quantified after exposure of specimens to different solvent ratios.

Keywords:

• artificial saliva
• bisphenol-A (BPA)
• bisphenol A glycerolate dimethacrylate (BIS-GMA)
• dental composites
• dimethacrylate monomers
• HPLC
• triethylene glycol dimethacrylate (TEGDMA)
• urethane dimethacrylate (UDMA)

Notes

Y = Peak area.

X = ng/µL.