SOLID PHASE EXTRACTION OF THREE PHENOLIC ACIDS FROM SALICONIA HERBACEA L. BY DIFFERENT IONIC LIQUID-BASED SILICAS

Abstract

Different ionic liquid-based silicas were prepared for solid-phase extraction of three phenolic acids (protocatechuic, ferulic, and caffeic) from Saliconia herbaces L. extraction solution, followed by high-performance liquid chromatography coupled to ultraviolet detection. By comparing the adsorption isotherms of the three phenolic acids on different ionic liquid-based silicas, the ionic interaction was shown as the dominant interaction with the highest adsorption capacity of phenolic acids obtained with the imidazolium silica. The data was then fitted into different adsorption isotherm equations and the Langmuir equation selected to optimize the loading volume in the of solid-phase extraction procedure. After washing with distilled water and elution with 30.0 mmol/L HCl solution, the phenolic acids were purified with the recovery yields of 94.69, 79.09, and 87.32% for protocatechuic acid, ferulic acid, and caffeic acid, respectively.

Keywords:

• HPLC
• ionic liquids
• phenolic acid
• Saliconia herbaces L.
• silica
• solid liquid extraction

ACKNOWLEDGMENT

This research was supported by an INHA University Research Grant (INHA-2012).