METHOD DEVELOPMENT FOR DETERMINATION OF CYAZOFAMID IN SOIL AND WATER BY HPLC

Abstract

A rapid, sensitive, and accurate method for analysis of cyazofamid has been developed using SPE in texturally different soils and water. The method involved using different solvents or their combinations for the extraction of fungicide from soil and water. The cleanup was done with C-18/florisil/alumina SPE cartridges and finally analyzed by high performance liquid chromatography (HPLC) using different columns, namely, C-18/diol/cyano and mobile phase using methanol/acetonitrile:water in different ratios with UV detection at 279 nm. The best results in terms of recovery, cleanup efficiency, and independence of soil characteristics were obtained with combination of acetone:methanol followed by cleanup with florisil for soil and chloroform for water. Most suited column was C-18 with mobile phase acetonitrile:water for HPLC. Calibration curve for quantification were linear (R² = 0.999) over the dynamic range of 0.1–10.0 µg mL⁻¹ and percent relative standard deviation (RSD) was 1.72%. The limit of detection for instrument and limit of quantification for method were 0.01 and 0.05 µg g⁻¹.

Keywords:

• cyazofamid
• HPLC
• method development
• soil
• SPE
• water

ACKNOWLEDGMENT

Authors are thankful to M/s Ishihara Sangyo Kaisha (ISK) Biosciences Corporation, Japan (through United Phosphorus Ltd, India) for providing analytical grade cyazofamid fungicide for research purpose. Ms. Nandita Singh is thankful to the Indian Council of Agriculture Research (ICAR), New Delhi, India for the financial support in the form of a Junior Research Fellowship and also acknowledges the university authorities and the department staff for providing necessary help during the research work.