CHIRAL HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC ANALYSIS OF ENANTIOMERS OF THREO-LOSIGAMONE

Abstract

A new liquid chromatographic method has been developed for the chiral separation of the enantiomers of threo-losigamone. The enantiomers were separated by a Chiralpak IB (250 × 4.6 mm, 5 µm) at 30°C and detected at 230 nm; the mobile phase used was n-hexane-ethanol (90/10, V/V) with a flow rate of 0.8 mL · min⁻¹. The resolution of threo-losigamone enantiomers was 1.79. By comparing the chromatographs of losigamone enantiomers and losigamone spiked with threo-losigamone, it was determined that the first effluent was threo-losigamone.

Keywords:

• chiralpak IB
• chiral stationary phase
• enantiomeric separation
• HPLC
• losigamone
• threo-losigamone

Notes

Note: Chromatographic condition: the column temperature was at 25°C, and the flow rate was 0.8 mL · min⁻¹.

“–” means that the separation was not seen.

Note: Chromatographic condition: mobile phase was n-hexane-ethanol (90/10, V/V), the flow rate was 0.8 mL · min⁻¹.

Note: Chromatographic condition: mobile phase was n-hexane-ethanol (90/10, V/V), the column temperature was at 25°C.